phlip icg Search Results


icg var3 phlip davinci si surgical system  (DaVinci Biosciences)
90
DaVinci Biosciences icg var3 phlip davinci si surgical system
Icg Var3 Phlip Davinci Si Surgical System, supplied by DaVinci Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phlip+icg/icg+var3+phlip+davinci+si+surgical+system/pm39798775-202-0-5
Average 90 stars, based on 1 article reviews
icg var3 phlip davinci si surgical system - by Bioz Stars, 2026-10
90/100 stars
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phlip icg  (Iris Biotech Gmbh)
86
Iris Biotech Gmbh phlip icg
Characterization of <t>pHLIP</t> <t>ICG</t> and blood clearance in mice and dogs. ( a ) The Var3 pHLIP sequence is shown with the chemical structure of the ICG dye coupled to a single Cys residue at the N-terminal part of pHLIP peptide. ( b ) Normalized absorbance (black line) and fluorescence (red line) spectra (the excitation is at 805 nm) of pHLIP ICG (5 µM) measured in DMF. ( c ) Circular dichroism (CD) spectra of pHLIP ICG measured in phosphate buffer (pH 8) in absence (pHLIP ICG, pH 8—black line) and presence (pHLIP ICG + PC, pH 8—blue line) of POPC liposomes, and in the presence of POPC liposomes at pH 5.2 (pHLIP ICG + PC, pH 5.2—red line) indicate on formation of helical structure in membrane at low pH. ( d ) pH-dependent insertion of pHLIP ICG into the bilayers of POPC liposomes monitored by changes of the CD spectral signal at 222 nm. The data were fitted with the Henderson-Hasselbach equation (red line with 95% confidence interval) to establish the midpoint of the transition (pK). ( e ) The kinetics signal (multiplied by − 1) of pHLIP ICG insertion into lipid bilayers of POPC liposomes as a result of a pH drop from pH 8 (blue line in insert) to pH 5 (red line in insert) was monitored in real-time by the tryptophan fluorescence changes (black line). The gray line is a baseline obtained by mixing pHLIP ICG with POPC liposomes at pH 8 with pH 8 buffer. The red line is a bi-exponential fit of the experimental data (85% of the signal changes occur with a rate of 5 s −1 and about 15% of final adjustment is completed at a rate of 0.077 s −1 ). ( f , g ) Blood clearance profile and single exponential fitting curves (red lines) obtained from mice ( f ) and dogs ( g ) after a single i.v. injection of 12.3 mg/kg of pHLIP ICG to mice and 0.064 mg/kg of pHLIP ICG to dogs are shown.
Phlip Icg, supplied by Iris Biotech Gmbh, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phlip+icg/icg+phlip/pmc07591906-25-15-25
Average 86 stars, based on 1 article reviews
phlip icg - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

Image Search Results


Characterization of pHLIP ICG and blood clearance in mice and dogs. ( a ) The Var3 pHLIP sequence is shown with the chemical structure of the ICG dye coupled to a single Cys residue at the N-terminal part of pHLIP peptide. ( b ) Normalized absorbance (black line) and fluorescence (red line) spectra (the excitation is at 805 nm) of pHLIP ICG (5 µM) measured in DMF. ( c ) Circular dichroism (CD) spectra of pHLIP ICG measured in phosphate buffer (pH 8) in absence (pHLIP ICG, pH 8—black line) and presence (pHLIP ICG + PC, pH 8—blue line) of POPC liposomes, and in the presence of POPC liposomes at pH 5.2 (pHLIP ICG + PC, pH 5.2—red line) indicate on formation of helical structure in membrane at low pH. ( d ) pH-dependent insertion of pHLIP ICG into the bilayers of POPC liposomes monitored by changes of the CD spectral signal at 222 nm. The data were fitted with the Henderson-Hasselbach equation (red line with 95% confidence interval) to establish the midpoint of the transition (pK). ( e ) The kinetics signal (multiplied by − 1) of pHLIP ICG insertion into lipid bilayers of POPC liposomes as a result of a pH drop from pH 8 (blue line in insert) to pH 5 (red line in insert) was monitored in real-time by the tryptophan fluorescence changes (black line). The gray line is a baseline obtained by mixing pHLIP ICG with POPC liposomes at pH 8 with pH 8 buffer. The red line is a bi-exponential fit of the experimental data (85% of the signal changes occur with a rate of 5 s −1 and about 15% of final adjustment is completed at a rate of 0.077 s −1 ). ( f , g ) Blood clearance profile and single exponential fitting curves (red lines) obtained from mice ( f ) and dogs ( g ) after a single i.v. injection of 12.3 mg/kg of pHLIP ICG to mice and 0.064 mg/kg of pHLIP ICG to dogs are shown.

Journal: Scientific Reports

Article Title: pHLIP ICG for delineation of tumors and blood flow during fluorescence-guided surgery

doi: 10.1038/s41598-020-75443-5

Figure Lengend Snippet: Characterization of pHLIP ICG and blood clearance in mice and dogs. ( a ) The Var3 pHLIP sequence is shown with the chemical structure of the ICG dye coupled to a single Cys residue at the N-terminal part of pHLIP peptide. ( b ) Normalized absorbance (black line) and fluorescence (red line) spectra (the excitation is at 805 nm) of pHLIP ICG (5 µM) measured in DMF. ( c ) Circular dichroism (CD) spectra of pHLIP ICG measured in phosphate buffer (pH 8) in absence (pHLIP ICG, pH 8—black line) and presence (pHLIP ICG + PC, pH 8—blue line) of POPC liposomes, and in the presence of POPC liposomes at pH 5.2 (pHLIP ICG + PC, pH 5.2—red line) indicate on formation of helical structure in membrane at low pH. ( d ) pH-dependent insertion of pHLIP ICG into the bilayers of POPC liposomes monitored by changes of the CD spectral signal at 222 nm. The data were fitted with the Henderson-Hasselbach equation (red line with 95% confidence interval) to establish the midpoint of the transition (pK). ( e ) The kinetics signal (multiplied by − 1) of pHLIP ICG insertion into lipid bilayers of POPC liposomes as a result of a pH drop from pH 8 (blue line in insert) to pH 5 (red line in insert) was monitored in real-time by the tryptophan fluorescence changes (black line). The gray line is a baseline obtained by mixing pHLIP ICG with POPC liposomes at pH 8 with pH 8 buffer. The red line is a bi-exponential fit of the experimental data (85% of the signal changes occur with a rate of 5 s −1 and about 15% of final adjustment is completed at a rate of 0.077 s −1 ). ( f , g ) Blood clearance profile and single exponential fitting curves (red lines) obtained from mice ( f ) and dogs ( g ) after a single i.v. injection of 12.3 mg/kg of pHLIP ICG to mice and 0.064 mg/kg of pHLIP ICG to dogs are shown.

Article Snippet: All manufacturing procedures were completed in accordance with good laboratory practice (GLP) quality control specifications. pHLIP ICG was synthesized and the agent was produced by Iris Biotech, GmbH (Germany) in partnership with Chemical and Biopharmaceutical Laboratories (CBL) (Greece) and then further optimized by CordenPharma.

Techniques: Sequencing, Residue, Fluorescence, Circular Dichroism, Liposomes, Membrane, Injection

Imaging of blood vessels in mice. Representative NIR pHLIP ICG fluorescent images of blood vessels at different time points after a single i.v. injection of ICG-Cys ( a ) or pHLIP ICG ( b ).

Journal: Scientific Reports

Article Title: pHLIP ICG for delineation of tumors and blood flow during fluorescence-guided surgery

doi: 10.1038/s41598-020-75443-5

Figure Lengend Snippet: Imaging of blood vessels in mice. Representative NIR pHLIP ICG fluorescent images of blood vessels at different time points after a single i.v. injection of ICG-Cys ( a ) or pHLIP ICG ( b ).

Article Snippet: All manufacturing procedures were completed in accordance with good laboratory practice (GLP) quality control specifications. pHLIP ICG was synthesized and the agent was produced by Iris Biotech, GmbH (Germany) in partnership with Chemical and Biopharmaceutical Laboratories (CBL) (Greece) and then further optimized by CordenPharma.

Techniques: Imaging, Injection

Imaging of blood vessels in pigs. Representative NIR pHLIP ICG (verification batch #1912127) fluorescent images and overlay of fluorescent and color images of blood vessels at different time points after injection of pHLIP ICG in PBS (dose 1—0.052 mg/kg; dose 2—additional 0.12 mg/kg; dose 3—additional 0.24 mg/kg) are shown. ( a ) Imaging inside the pig body cavity was performed using a Stryker 1688 AIM system (808 nm excitation) for endoscopic/laparoscopic imaging (gain 8/10 for dose 2, gain 7/10 for dose 3). ( b ) Imaging of a pedicle flap was performed using a Stryker SPY-PHI handheld device (805 nm excitation) for open-field imaging.

Journal: Scientific Reports

Article Title: pHLIP ICG for delineation of tumors and blood flow during fluorescence-guided surgery

doi: 10.1038/s41598-020-75443-5

Figure Lengend Snippet: Imaging of blood vessels in pigs. Representative NIR pHLIP ICG (verification batch #1912127) fluorescent images and overlay of fluorescent and color images of blood vessels at different time points after injection of pHLIP ICG in PBS (dose 1—0.052 mg/kg; dose 2—additional 0.12 mg/kg; dose 3—additional 0.24 mg/kg) are shown. ( a ) Imaging inside the pig body cavity was performed using a Stryker 1688 AIM system (808 nm excitation) for endoscopic/laparoscopic imaging (gain 8/10 for dose 2, gain 7/10 for dose 3). ( b ) Imaging of a pedicle flap was performed using a Stryker SPY-PHI handheld device (805 nm excitation) for open-field imaging.

Article Snippet: All manufacturing procedures were completed in accordance with good laboratory practice (GLP) quality control specifications. pHLIP ICG was synthesized and the agent was produced by Iris Biotech, GmbH (Germany) in partnership with Chemical and Biopharmaceutical Laboratories (CBL) (Greece) and then further optimized by CordenPharma.

Techniques: Imaging, Injection

Tumor targeting. Representative photos and overlay of photos and in vivo NIR pHLIP ICG fluorescent images of athymic female nude mice bearing murine and human tumors are shown with skin removed from the tumor site. Targeting of human breast MDA-MB-231 tumor ( a ), murine breast 4T1 tumor ( b ), human A549 lung tumor ( c ), murine LLC Lewis lung carcinoma ( d ), human M4A4 epithelial tumor ( e ), human HeLa cervical tumor ( f ), human UMUC3 urinary bladder tumor ( g ) and human prostate LNCaP tumor ( h ) are shown. The imaging was performed at 24 h after a single i.v. administration of pHLIP ICG. Arrows indicate tumor locations. In the case of the MDA-MB231 breast tumor model, both a small tumor (arrow at the right) and an inguinal lymph node (indicated as LN) are targeted.

Journal: Scientific Reports

Article Title: pHLIP ICG for delineation of tumors and blood flow during fluorescence-guided surgery

doi: 10.1038/s41598-020-75443-5

Figure Lengend Snippet: Tumor targeting. Representative photos and overlay of photos and in vivo NIR pHLIP ICG fluorescent images of athymic female nude mice bearing murine and human tumors are shown with skin removed from the tumor site. Targeting of human breast MDA-MB-231 tumor ( a ), murine breast 4T1 tumor ( b ), human A549 lung tumor ( c ), murine LLC Lewis lung carcinoma ( d ), human M4A4 epithelial tumor ( e ), human HeLa cervical tumor ( f ), human UMUC3 urinary bladder tumor ( g ) and human prostate LNCaP tumor ( h ) are shown. The imaging was performed at 24 h after a single i.v. administration of pHLIP ICG. Arrows indicate tumor locations. In the case of the MDA-MB231 breast tumor model, both a small tumor (arrow at the right) and an inguinal lymph node (indicated as LN) are targeted.

Article Snippet: All manufacturing procedures were completed in accordance with good laboratory practice (GLP) quality control specifications. pHLIP ICG was synthesized and the agent was produced by Iris Biotech, GmbH (Germany) in partnership with Chemical and Biopharmaceutical Laboratories (CBL) (Greece) and then further optimized by CordenPharma.

Techniques: In Vivo, Imaging

Ex vivo imaging of tumors with surrounding muscle. Representative photos, and overlays of photos and ex vivo NIR pHLIP ICG fluorescent images of tumors resected with surrounding tissue immediately after in vivo imaging. Targeting of human breast MDA-MB-231 tumor ( a ), murine breast 4T1 tumor ( b ), human A549 lung tumor ( c ), murine LLC Lewis lung carcinoma ( d ), human M4A4 epithelial tumor ( e ), human HeLa cervical tumor ( f ), human UMUC3 urinary bladder tumor ( g ) and human prostate LNCaP tumor ( h ) are shown. In the case of the MDA-MB231 breast tumor model, both a small tumor and an inguinal lymph node (indicated as LN) are targeted.

Journal: Scientific Reports

Article Title: pHLIP ICG for delineation of tumors and blood flow during fluorescence-guided surgery

doi: 10.1038/s41598-020-75443-5

Figure Lengend Snippet: Ex vivo imaging of tumors with surrounding muscle. Representative photos, and overlays of photos and ex vivo NIR pHLIP ICG fluorescent images of tumors resected with surrounding tissue immediately after in vivo imaging. Targeting of human breast MDA-MB-231 tumor ( a ), murine breast 4T1 tumor ( b ), human A549 lung tumor ( c ), murine LLC Lewis lung carcinoma ( d ), human M4A4 epithelial tumor ( e ), human HeLa cervical tumor ( f ), human UMUC3 urinary bladder tumor ( g ) and human prostate LNCaP tumor ( h ) are shown. In the case of the MDA-MB231 breast tumor model, both a small tumor and an inguinal lymph node (indicated as LN) are targeted.

Article Snippet: All manufacturing procedures were completed in accordance with good laboratory practice (GLP) quality control specifications. pHLIP ICG was synthesized and the agent was produced by Iris Biotech, GmbH (Germany) in partnership with Chemical and Biopharmaceutical Laboratories (CBL) (Greece) and then further optimized by CordenPharma.

Techniques: Ex Vivo, Imaging, In Vivo Imaging

Ex vivo imaging and imaging of sections. ( a ) Representative images of tumors with surrounding tissue: photos, NIR pHLIP ICG fluorescent images, overlay of photos with NIR pHLIP ICG fluorescent images, and images of HE-stained sections obtained from the same tumor-muscle pieces are shown. ( b ) Bright field image of HE-stained section and NIR pHLIP ICG fluorescent images of the adjacent non-processed section obtained on IR scanner shown in monochrome (green) and 16-color (white and red are the highest intensity, and black and dark blue are the lowest intensity) presentations. T indicates tumor; M indicates muscle, and S indicates stroma. ( c ) Magnified bright field images (using 20 × objective) of tumor (T), muscle (M1 and M2), tumor stroma (TS1, TS2 and TS3) of HE-stained section are presented in panel ( b ).

Journal: Scientific Reports

Article Title: pHLIP ICG for delineation of tumors and blood flow during fluorescence-guided surgery

doi: 10.1038/s41598-020-75443-5

Figure Lengend Snippet: Ex vivo imaging and imaging of sections. ( a ) Representative images of tumors with surrounding tissue: photos, NIR pHLIP ICG fluorescent images, overlay of photos with NIR pHLIP ICG fluorescent images, and images of HE-stained sections obtained from the same tumor-muscle pieces are shown. ( b ) Bright field image of HE-stained section and NIR pHLIP ICG fluorescent images of the adjacent non-processed section obtained on IR scanner shown in monochrome (green) and 16-color (white and red are the highest intensity, and black and dark blue are the lowest intensity) presentations. T indicates tumor; M indicates muscle, and S indicates stroma. ( c ) Magnified bright field images (using 20 × objective) of tumor (T), muscle (M1 and M2), tumor stroma (TS1, TS2 and TS3) of HE-stained section are presented in panel ( b ).

Article Snippet: All manufacturing procedures were completed in accordance with good laboratory practice (GLP) quality control specifications. pHLIP ICG was synthesized and the agent was produced by Iris Biotech, GmbH (Germany) in partnership with Chemical and Biopharmaceutical Laboratories (CBL) (Greece) and then further optimized by CordenPharma.

Techniques: Ex Vivo, Imaging, Staining